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TAP MediumTAP Medium Directions For 2 L Total To approximately 1600 mL of dH2O, add each of the components in the order specified while stirring continuously. Bring the total volume to 2 L with dH2O. For 1. 5% agar medium add 30 grams of agar into the flask; do not mix. Cover and autoclave medium. Allow to cool and store at refrigerator temperature. For agar medium, dispense before agar solidifies. # Component Amount Stock Solution Concentration Final
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TAP Medium

Directions

For 2 L Total
  1. To approximately 1600 mL of dH2O, add each of the components in the order specified while stirring continuously.
  2. Bring the total volume to 2 L with dH2O.
    • For 1.5% agar medium add 30 grams of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. Allow to cool and store at refrigerator temperature.
    • For agar medium, dispense before agar solidifies.

# Component Amount Stock Solution Concentration Final Concentration
1 Beijerinck's Solution 100 mL/2 L
2 Phosphate Buffer Stock Solution 17 mL/2 L
3 Hunter's Trace Stock Solution 2 mL/2 L
4 Tris Acetate Stock Solution 20 mL/2 L

TAP Medium

Item no : 17408779855
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