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Polytomella MediumPolytomella Medium Suitable for all axenic cultures of Polytoma, Polytomella and Astasia, the colorless strains of Euglena and 2016 Scenedesmus obliquus. Directions For 1 L Total To approximately 950 mL of dH2O, add each of the components in the order specified while stirring continuously. Bring total volume to 1 L with dH2O. For 1. 5% agar medium add 15 grams of agar into the flask; do not mix. Cover and autoclave medium. Store at refrigerator
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Polytomella Medium

Suitable for all axenic cultures of Polytoma, Polytomella and Astasia, the colorless strains of Euglena and 2016 Scenedesmus obliquus.

Directions

For 1 L Total
  1. To approximately 950 mL of dH2O, add each of the components in the order specified while stirring continuously.
  2. Bring total volume to 1 L with dH2O.
    • For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Yeast extract
(Bacto, Difco)
1 g/L
2 Tryptone
(Sigma T 9410)
1 g/L
3 Sodium acetate
(Fisher BP 333)
2 g/L 24.4 mM

Polytomella Medium

Item no : 24120393208
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