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Soil+Seawater MediumSoil+Seawater Medium Directions For marine algae that require salinities at 30 ppt. For 200 mL Total Combine components. Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95 C in 15 minutes. Then increased just over 98 C for the 3 hour duration. Cooling occurs gradually at room temperature. Refrigerate 24 hours or more and bring to room temperature
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Soil+Seawater Medium

Directions

For marine algae that require salinities at 30 ppt.

For 200 mL Total
  1. Combine components.
  2. Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95 °C in 15 minutes. Then increased just over 98 °C for the 3 hour duration. Cooling occurs gradually at room temperature.
  3. Refrigerate 24 hours or more and bring to room temperature before use.

# Component Amount Stock Solution Concentration Final Concentration
1 Green House Soil 1 tsp/200 mL of Seawater
2 Pasteurized Seawater 200 mL
2 CaCO3
(optional) (Fisher C 64)
1 mg/200 mL of Seawater 0.05 mM

Soil+Seawater Medium

Item no : 35122588114
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