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Volvox-Dextrose MediumVolvox Dextrose Medium Directions For 1 L Total Prepare 1 L of Volvox Medium, except add 10 grams of glucose after all other stock solutions have been added. Bring total volume to 1 L with dH2O. For 1. 5% agar medium add 15 grams of agar into the flask; do not mix. Cover and autoclave medium. When cooled add vitamins. For agar medium add vitamins, mix, and dispense before agar solidifies. # Component Amount Stock Solution Concentration Final
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Volvox-Dextrose Medium

Directions

For 1 L Total
  1. Prepare 1 L of Volvox Medium, except add 10 grams of glucose after all other stock solutions have been added.
  2. Bring total volume to 1 L with dH2O.
    • For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. When cooled add vitamins.
    • For agar medium add vitamins, mix, and dispense before agar solidifies.

# Component Amount Stock Solution Concentration Final Concentration
1 Volvox Medium 1 L
2 Glucose
(BACTO-dextrose or Sigma D 9634)
10 g/L 0.06 M
3 Vitamin B12 1 mL/L
4 Biotin Vitamin Solution 1 mL/L

Volvox-Dextrose Medium

Item no : 49176354768
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