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N/20 MediumN 20 Medium Directions For 1 L Total To approximately 850 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously. Bring total volume to 1 L with dH2O. Cover and autoclave medium. When cooled, add vitamins. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 KH2PO4 (Sigma P 0662) 2 mL L 17. 5 g 200 mL dH2O 1. 29 mM 2 NaNO3 (Fisher BP360 500) 100 L
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N/20 Medium
Directions
For 1 L Total
- To approximately 850 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
- Bring total volume to 1 L with dH2O.
- Cover and autoclave medium.
- When cooled, add vitamins.
- Store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | KH2PO4 (Sigma P 0662) |
2 mL/L | 17.5 g/200 mL dH2O | 1.29 mM |
| 2 | NaNO3 (Fisher BP360-500) |
100 µL/L | 25 g/200 mL dH2O | 0.14 mM |
| 3 | K2HPO4 (Sigma P 3786) |
1 mL/L | 15 g/200 mL dH2O | 0.43 mM |
| 4 | NaCl (Fisher S271-500) |
1 mL/L | 5 g/200 mL dH2O | 0.43 mM |
| 5 | CaCl2•2H2O (Sigma C-3881) |
1 mL/L | 5 g/200 mL dH2O | 0.17 mM |
| 6 | MgSO4•7H2O (Sigma 230391) |
1 mL/L | 15 g/200 mL dH2O | 0.3 mM |
| 7 | P-IV Metal Solution | 6 mL/L | ||
| 8 | Sodium acetate (Fisher BP 333) |
0.67 g/L | 8.12 mM | |
| 9 | TES buffer (Sigma T 1375) |
0.92 g/L | 4.01 mM | |
| 10 | Vitamin B12 | 1 mL/L | ||
| 11 | Thiamine Vitamin Solution | 1 mL/L |
N/20 Medium
Item no
:
62582614603
US$ 55.00
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1piece
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Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
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- Standard Shipping : 3-10 business days
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
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