Soilwater: GR+ Medium

The basic garden-type soilwater; includes a pinch of CaCO₃, which is added to the soil and water prior to steaming; suitable for most phototrophic freshwater algae. Optional ingredients: add Vitamin B₁₂ to cultures of Volvox; a pinch of NH₄MgPO₄•6H₂O added to soilwater cultures of Botryococcus, Synechococcus and some Euglenoids enhances growth and to LB 826 Gonium pectorale increases the numbers of 16-celled colonies formed.

Directions

For 200 mL Total

1. Combine components.
2. Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95 °C in 15 minutes. Then increased just over 98 °C for the 3 hour duration. Cooling occurs gradually at room temperature.
3. Refrigerate 24 hours or more and bring to room temperature before use.