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8 ppt F/2 Medium8 ppt F 2 Medium Directions For 1 L Total Dilute 1 Liter of seawater to 8 ppt. To approximately 950 mL of the non pasteurized seawater (8 ppt), add each of the components in the order specified (except vitamins) while stirring continuously. Bring total volume to 1 L with remaining non pasteurized seawater (8 ppt). For 1. 5% agar medium add 15 g of agar into the flask; do not mix. Cover and autoclave medium. When cooled add sterile vitamins. For agar
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8 ppt F/2 Medium
Directions
For 1 L Total
- Dilute 1 Liter of seawater to 8 ppt.
- To approximately 950 mL of the non-pasteurized seawater (8 ppt), add each of the components in the order specified (except vitamins) while stirring continuously.
- Bring total volume to 1 L with remaining non-pasteurized seawater (8 ppt).
- For 1.5% agar medium add 15 g of agar into the flask; do not mix.
- Cover and autoclave medium.
- When cooled add sterile vitamins.
- For agar medium add vitamins, mix, and dispense before agar solidifies.
- Store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | NaNO3 (Fisher BP360-500) |
1 mL | 7.5 g/100 mL dH20 | 880 µM |
| 2 | NaH2PO4•H2O (MCIB 742) |
1 mL | 0.5 g/100 mL dH20 | 36 µM |
| 3 | Na2SiO3•9H2O (Sigma 307815) |
1 mL | 3 g/100 mL dH20 | 106 µM |
| 4 | Trace Metals Solution | 1 mL/L | ||
| 5 | Vitamin B12 | 1 mL/L | ||
| 6 | Biotin Vitamin Solution | 1 mL/L | ||
| 7 | Thiamine Vitamin Solution | 1 mL/L |
8 ppt F/2 Medium
Item no
:
93388386282
US$ 60.00
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