A+ Medium

Directions

For 1 L Total

Liquid media:

1. To approximately 900 mL of dH₂O add the first 8 components in the order specified while stirring continuously.
2. Bring total volume to 1 L with dH₂O.
3. Cover and autoclave medium.
4. Add filter sterilized A+ Trace Components.
5. Allow to cool then store at refrigerator temperature.

Agar media:

1. To approximately 390 mL of dH₂O add the first 8 components in the order specified while stirring continuously.
2. Bring total volume to 500 mL with dH₂O.
3. In a separate container add 15 g of agar to 500 mL of dH₂O (Final 1.5% w/v).
4. Cover and autoclave both solutions.
5. In a water bath allow both solutions to cool to 45-50 °C.
6. Add sterile Sodium Thiosulfate to agar solution and mix well.
7. Add filter sterilized A+ Trace Components.
8. Combine both agar and liquid solutions, mix well. Note the agar can solidify quickly.
9. Allow to cool then store at refrigerator temperature.