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MES-Volvox MediumMES Volvox Medium General purpose medium for freshwater strains, especially those requiring ammonium. Suitable for xenic and axenic cultures. Modified Volvox Medium. Directions For 1 L Total To approximately 950 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously. Adjust the pH to 6. 7 Bring the total volume to 1 L with dH2O. For 1. 5% agar medium add 15 grams of agar into the flask; do not mix.
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MES-Volvox Medium
General purpose medium for freshwater strains, especially those requiring ammonium. Suitable for xenic and axenic cultures. Modified Volvox Medium.
Directions
For 1 L Total
- To approximately 950 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
- Adjust the pH to 6.7
- Bring the total volume to 1 L with dH2O.
- For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
- Cover and autoclave medium.
- When cooled, add vitamins.
- For agar medium add vitamins, mix, and dispense before agar solidifies.
- Store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration Per 1 Liter |
|---|---|---|---|---|
| 1 | Ca(NO3)2•4H2O (CAS: 13477-34-4) |
1 mL/L | 11.8 g/100 mL dH20 | 0.5 mM |
| 2 | MgSO4•7H2O (CAS: 10034-99-8) |
1 mL/L | 4 g/100 mL dH20 | 0.16 mM |
| 3 | Na2glycerophosphate•5H2O (CAS: 13408-09-8) |
1 mL/L | 5 g/100 mL dH20 | 0.16 mM |
| 4 | KCl (CAS: 7447-40-7) |
1 mL/L | 5 g/100 mL dH20 | 0.67 mM |
| 5 | MES (CAS: 1266615-59-1) |
1.95 g/L | 10 mM | |
| 6 | P-IV Metal Solution | 6 mL/L | ||
| 7 | NH4Cl (CAS: 12125-02-9) |
1 mL/L | 2.67 g/100 mL dH20 | 0.5 mM |
| 8 | Vitamin B12 | 1 mL/L | ||
| 9 | Biotin Vitamin Solution | 1 mL/L |
MES-Volvox Medium
Item no
:
35643182508
US$ 55.00
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